Combined AFM / fluorescent microscopy

An integrated AFM and optical microscope for bioscience research.

This system performs the same as any AFM, but with the added benefit of fluorescent sample imaging which can be co-localized to AFM region of interest.

The IX83 microscope base allows for in situ fluorescent microscopy along with AFM characterization

Objectives: 10x (NA 0.3), 40x (NA 0.75), 60x (NA 0.9)

Fluorescent Cubes: DAPI, EGFP (FITC/Cy2), mCherry/Texas Red, Cy5

Fluorescent Source: X-Cite Turbo Multi Wavelength (XT600-T)

Camera: Hamamatsu C13440 (Instructions Manual)

Camera 2: Pixel Link PL-D721CU

Standard AFM

The highest-performing member of the MFP-3D family.

This system has the widest range of imaging modes and accessories for routine imaging and exploratory research.

Custom Probe Fabrication

The micromanipulator probe station allows for custom probe fabrication.

Examples of modification include, single bacterium, pollen particle, or microsphere tip creation.

Fluorescent Microscopy

This IX83 microscope allows for fluorescent and phase contrast microscopy

Objectives: 5x (NA 0.13, PhL), 10x (0.30, Ph1), 20x (0.50, Ph1), 40x (NA 0.75, Ph2), 100x (Oil, NA 1.30, Ph3)

Fluorescent Cubes: DAPI 5050C, FITC 5050A, TRITC 8, Cy5 4040C

Fluorescent Source: X-cite Exacte (Mercury Arc lamp)

Camera: Q-Imaging Retiga 6000

The Closed Fluid Cell (CFC) for MFP-3D AFMs is designed to hold gases or liquids either statically or with perfusion through configurable inlet/outlet ports. The unique design enables the cell, sample, and cantilever holder to be fully assembled and sealed in a controlled environment (e.g. glovebox or hood) and then transferred to the AFM for use. Two related versions are available: 1) the “Fluid Cell Lite” that has no inlet/outlet ports and minimizes evaporation but is not completely sealed, and 2) the “Electrical Closed Cell,” which enables electrical connections to the sample.

• Completely enclosed and sealed cell
• Ten access ports for fluid for gas exchange
• Easy cleaning prevents contamination
• Accommodates a variety of sample mounts

Data Sheet

The BioHeater is similar in design to the Closed Fluid Cell except that an integrated immersion heating element and temperature sensor are positioned in the fluid volume, allowing imaging in fluid between ambient and 80°C. It supports samples up to 25 mm in diameter. The kit includes various sample accessories and spare parts, as well as a membrane and clamp for sealed operation. (Compatible with all MFP-3D AFMs except Origin.)

• Heats samples in liquids from ambient to 80°C
• Ten ports are available—seven for liquid and gas exchange and electrical access, and three used by the heating element
• Easy disassembly for cleaning

Data Sheet

The Humidity Sensing Cell measures humidity conditions with a sensor located within a sealed sample cell. The cell supports samples up to 30 mm in diameter. In addition, a moat that surrounds the sample with a salt solution allows for humidity control. The kit includes various sample accessories, as well as a membrane and clamp for sealed operation (compatible with all MFP-3D AFMs except Origin).

• Small sealed sample chamber with a dedicated side access port for the solid state humidity sensor
• Humidity control can be accomplished by filling a cup with concentrated salt solutions or by flowing a mixture of dry and wet gas through one of the four access ports

Data Sheet

Ztherm combines powerful conventional and new advanced thermal analysis capabilities with the high resolution of AFM, and adds patent pending compensation for thermally-induced cantilever bending and measurement of contact stiffness and dissipation for the highest sensitivity and resolution available. The Ztherm Option provides highly localized heating with sensitivity to ≤10-22 liter (sub-zeptoliter) materials property changes, more than an order of magnitude improvement in volume over that previously available with commercial systems.

Data Sheet

The Humidity Sensing Cell measures humidity conditions with a sensor located within a sealed sample cell. The cell supports samples up to 30 mm in diameter. In addition, a moat that surrounds the sample with a salt solution allows for humidity control. The kit includes various sample accessories, as well as a membrane and clamp for sealed operation (compatible with all MFP-3D AFMs except Origin).

• Small sealed sample chamber with a dedicated side access port for the solid state humidity sensor
• Humidity control can be accomplished by filling a cup with concentrated salt solutions or by flowing a mixture of dry and wet gas through one of the four access ports

Data Sheet

The Environmental Controller interfaces with the BioHeater™, PolyHeater™, CoolerHeater, Petri Dish Heater, and Humidity Sensing Cell to provide unprecedented precision and accuracy. The Environmental Controller is sold separately but works with multiple accessories using the SmartStart™ interface to auto-configure each for easy plug and play operation.

• Unprecedented precision and accuracy
• SmartStart auto-configures for any accessory for plug and play operation

Data Sheet